PROFILES OF IONTOPHORETIC TRANSPORT OF NANOPARTICLES ACROSS IN VITRO RABBIT CORNEA
Jae Yeon Lee1, Se-Na Kim1,2, Young Bin Choy1,3,
1Seoul National University, South Korea;
2Harvard– MIT Program of Health Sciences and Technology, USA;
3Institute of Medical & Biological Engineering, Medical Research Center, Seoul National University, South Korea
Iontophoresis is a non-invasive technique used to transport substances of interest across tissues and this has drawn interest in ophthalmic fields to enhance delivery efficiency of topically administered drugs. Thus, there has been several trials to deliver small molecules across cornea into the eye tissue using iontophoresis; however, few were reported to transport the nanoparticles. The purpose of this study was, therefore, to profile an iontophoretic transport of the nanoparticles made of a biodegradable polymer, poly(lactic-co-glycolic acid) (PLGA), where we varied the conditions of iontophoresis applied via the cornea of in vitro rabbit eyes. For this, we applied the formulation of fluorescence-tagged PLGA nanoparticles onto the cornea and varied the factors, such as the size of nanoparticles, amplitude of electric current and time for iontophoresis application. After the iontophoretic application of the nanoparticles, we observed the fluorescence intensity of each of the cross-sections of the cornea with confocal fluorescence microscopy to assess the distribution of PLGA nanoparticles. The work is now in progress to mathematically model the iontophoretic movement of PLGA nanoparticles into the cornea.