CYTOSKELETAL DYNAMICS OF HUMAN RED BLOOD CELLS AND IMPLICATIONS IN PLASMODIUM INFECTION
Rajesh Chandramohanadas1,Trang Chu1, Ameya Sinha1, Benoit Mallerett2, Bruce Russell3, Laurent Renia2
1Singapore University of Technology & Design, Singapore;
2A*STAR; 3National University of Singaopore, Singapore
Human red blood cells (RBCs) undergo remarkable morphological, biochemical and bio-physical transformations during maturation. Using a combination of bio-physical and bio-chemical techniques, we have documented the global differences in red cells, along their maturation from young reticulocytes to mature erythrocytes. Our data revealed comparable abundance for the majority of membrane proteins between reticulocytes and normocytes. However, higher abundance of a selected set of structural components, such as β- tubulin and talin-1/2 in reticulocytes and their loss upon maturation was observed and validated. These proteins could contribute to unique mechanical properties and nanostructure of the young reticulocytes. Furthermore, how infectious agents such as Plasmodium, manipulates the RBC cytoskeleton during asexual infectious cycle will also be discussed.