ENGINEERED BONE TISSUES: FROM REGENERATIVE MEDICINE TO DISEASE MODELS
Nanyang Technological University, Singapore
Tissue engineering has traditionally been applied in the field of the regenerative medicine. Our group and others have reported that such approaches may be used to generate vascularized osseous tissue in vitro and further demonstrated their utility in the treatment of large fractures. Following on the observation that human tissue engineered bone (hTEB) recapitulate many physiological processes, we asked the question whether a similar strategy could be applied towards engineering biological models of diseased state. This talk will cover three platforms that are being developed in our group for the study of bone metastatic disease: (i) HuMice (Humanised Mice), where mice engrafted with hTEB provide systemic readouts (ii) BOnEMets (Bioreactors for the Observation aNd Evaluation of Metastasis), where macrotissue models of bone metastatic lesions are subjected to defined chemical and physical stimuli and finally, (iii) MiMiC (Migration and Metastasis in a Chip), where bone microenvironments are engineered onto microfluidic chips to study migration and extravasation events at single-cell levels.