ISOLATION OF NEW DRUG TARGETS WITH AN INNOVATIVE PROTEIN EXTRACTION TECHNOLOGY
Seth Hanson1, Saikat Nandi2
1Gilson, Inc., USA;
2Cold Spring Harbor Laboratory, USA
Co-immunoprecipitation (Co-IP) is a common technique for observing protein-protein interactions; however, weakly- or transiently-bound species often go undetected. Aggressive or repeated wash steps in the Co-IP process can result in a loss of these interactions. Exclusion-based sample preparation (ESPTM) allows for rapid yet gentle washing of protein solutions during Co-IP, thereby creating an environment that retains these elusive interactions. In the presentation I will summarize recent progress in developing EXTRACTMAN®, an innovative biomolecule extraction platform that enables researchers to better study complex protein interactions that are critical for drug target identification and a wide variety of topics in proteomic research. I will also describe a comparison of a conventional Co-IP protocol with an EXTRACTMAN® protocol using ESPTM technology. While the conventional Co-IP protocol did not pull down the protein-protein complex, EXTRACTMAN® did, demonstrating the utility of ESPTM technology and EXTRACTMAN®.