ACCURATE AND EFFICIENT TREATMENT OF TUMOR WITH OPTICAL COHERENCE TOMOGRAPHY-GUIDED LASER THERAPY
Wei-Chuan Chen1, Hsin-Yi Chou1, Chun-Chieh Wang1,2, Meng-Tsan Tsai1
1Chang Gung University, Taiwan;
2Chang Gung Memorial Hospital, Taiwan
Laser therapy has been widely implemented for various biomedical applications such as ophthalmology, dermatology, dentistry, and ENT. However, laser therapy may induce additional damages on the surrounding tissue and it is difficult to accurately identify the treatment region. In this study, we proposed to develop optical coherence tomography (OCT)-guided laser therapy for identification of lesion and real-time monitoring the treatment procedure as well as evaluation of tissue recovery. OCT enables reconstruct the microstructures of biological tissue without extra contrast agent. A functional OCT system was developed to simultaneously obtain the 3D structures and angiography of biological tissue. Moreover, a CW laser at 455 nm was integrated with the developed OCT system to induce tissue ablation. The developed OCT system can provide an axial and transverse resolutions of 7 mm and 5 mm respectively, enabling to differentiate the tumor development in the early-stage. In this study, the tumor cells were implanted in the skin of mice and the mice skin was scanned and treated by the integrated OCT-guided laser therapy system. The results showed that the tumor can be 3D visualized and the tumor region can be accurately exposed by the CW laser with minimized additional damages. Moreover, the treatment outcome can be in vivo monitored and evaluated. The results demonstrate that such OCT-guided laser therapy system can improve the treatment efficiency and reduce the additional damages, potentially enabling to implement for various biomedical applications.
Keywords: optical coherence tomography, laser ablation, tumor, imaging-guided therapy, angiography