DEVELOPMENT AND BIOLOGICAL EVALUATION OF A SPRAYING DEVICE FOR AUTOLOGOUS PLATELET-RICH BLOOD DERIVATIVES
Katharina Düregger, Alexander Gäble, Sabine Wacker, Markus Eblenkamp
Institute of Medical and Polymer Engineering, Technical University of Munich, Germany
Autologous Platelet-Rich Blood Derivatives are used in a variety of medical fields, such as sports medicine, orthopedic surgeries and wound healing. Especially the enhancement of wound healing by applying growth factors released from platelets is a promising application. Chronic wounds often affect large areas and treating the whole surface with autologous plateletrich blood derivatives can be achieved by spraying. Platelet activation during spraying should be held low to allow a physiological release of growth factors at the wound site.
In this study we present a compact, self-contained, and aseptic spraying device without any external connections such as a power supply or compressor. The biocompatible propellant R134a (1, 1, 1, 2-tetrafluoroethane) is used to aspirate and atomize the blood derivatives, which can be filled into a contained reservoir via a three-way valve. A twosubstance nozzle designed for production using Multi Jet Modeling (MJM) functions as the operating unit, throttle, mixing zone and atomization unit. The valve of the aerosol can is directly connected to the nozzle enabling the release of R134a when operated. A diameter reduction of the propellant channel adjusts the inlet pressure using the Venturi principle and enables self-priming conditions for the blood derivative. The blood reservoir is connected to the nozzle by a flexible tube.
Biological evaluation was determined by flow cytometry comparing platelet activation in the blood derivatives before and after spraying. Scanning electron microscopy was used to determine the distribution of the platelets in the spraying process and the morphology of the sprayed blood cells. The results indicate that the developed spraying device has low mechanical impact on the cellular components of the blood derivatives and is a promising tool for applying evenly distributed platelet-rich blood derivatives to large wound sites.