A NOVEL COATING TECHNIQUE FOR IMPROVING IN-VIVO BIOAFFINITY OF IMPLANT MEDICAL DEVICES
Daisuke Yoshino1, Masaaki Sato2
1Institute of Fluid Science, Tohoku University, Japan;
2Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Japan
Lifestyle-related disease, leading to arteriosclerosis, chronic kidney disease, and so on, becomes a serious problem in the aging society. Many kinds of implant medical devices are required for treatments of those diseases. Although cytotoxicity and biocompatibility of materials used in the devices have been evaluated, their bioaffinity, which indicates high cellular adhesiveness and tissue connectivity, has been treated as unimportant. Recently, surface modification by plasma or chemical processes has been used to improve bioaffinity of the implant devices. However, there are a lot of problems, for examples: these methods take cumbersome procedure; the target and the biological molecules possible to use for modification are limited. Considering these problems, we developed a novel coating technique of biological molecules for improving in-vivo bioaffinity of the implant devices. We can easily coat a biological molecule onto the surface of the target without the surface modification by treating and excessively charging an aqueous solution of the molecule with plasma discharge. We also verified the mechanism coating the biological molecule onto the target surface using measurement of contact angle of the treated solution. Based on the measurement of the contact angle, a mode of wettability transits from “adhesion wetting” to “immersional wetting” because of change in the surface tension of the solution charged with the plasma discharge. Consequently, the target surface can efficiently get a wetting for the solution of the biological molecule. Using the technique developed in this study, we evaluated its effect on cellular adhesiveness to a hydrophobic silicone coated cover glass. In the case of coating the hydrophobic cover glass with 50 µg/ml collagen solution, the developed technique led to significant increases in cellular adhesiveness and proliferation in comparison with coating of non-treated collagen solution.