A5: Cell & Molecular Mechanobiology I

CURVATURE EFFECT ON MONOLAYER CELL MIGRATION

Wang Xi1,2, Surabhi Sonam2,3, Benoit Ladoux2,4, Chwee Teck Lim1,2,3,5

1Graphene Research Centre, National University of Singapore, Singapore;
2Mechanobiology Institute, National University of Singapore, Singapore;
3National University of Singapore, Singapore;
4Institut Jacques Monod, Université Paris Diderot & CNRS, France;
5Singapore MIT Alliance Research and Technology, National University of Singapore, Singapore

In vivo cell sheets constitute a variety of curved architectures such as lung alveoli, kidney nephron and breast acini. Notably, during certain organ development and wound healing processes, cell monolayers often advanced on a curved surface. While a considerable amount of effort has been made to study monolayer cell migration, most of the studies have primarily used 2D-cell culture systems, which fail to recapitulate the complex geometric nature of tissues and organs. The mechanistic basis of cell behavior on a 3D curvature also remains poorly understood. Here, we investigate monolayer cell migration on various curved surfaces. We study intra-cellular arrangements, cell polarity and cell sheet velocity. Our findings reveal interesting insights into migration of cell monolayer in response to extent of curvature and can assist in better understanding tissue morphogenesis.

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